محافظت ویتامین از شرایط سیستم گوارش با استفاده از میکروژل آلژینات- پروتئین آب پنیر. مطالعه موردی ویتامین B کمپلکس

نوع مقاله : مقاله پژوهشی لاتین

نویسنده

• محسن زندی

گروه علوم و مهندسی صنایع غذایی، دانشگاه زنجان، دانشکده کشاورزی، گروه علوم و مهندسی صنایع غذائی.

https://doi.org/10.22067/ifstrj.v16i3.79215

چکیده

کمبود ویتامین اخیراً در برخی از کشورها به سبب رژیم غذایی نامتعادل یا ناقص وجود دارد، از این‌رو غنی‌سازی مواد غذایی با ویتامین ضروری می‌باشد. محافظت ویتامین در میکروژل سبب افزایش پایداری و زیست فراهمی عوامل فعال در برابر شرایط سیستم گوارش می‌گردد. هدف تحقیق اخیر تعیین، مقایسه و توسعه سیستم تحویل ایده‌آل به‌منظور محافظت ویتامین در برابر شرایط گوارش می‌باشد. برای این منظور، میکروژل آلژینات- پروتئین آب پنیر حاوی ویتامین به‌عنوان حامل بیوپلیمری ایجاد و توسعه یافت. این میکروکپسول از منظر مورفولوژی، اندازه‌گیری پتانسیل زتا، اندازه‌گیری توزیع اندازه ذرات، راندمان انکپسولاسیون و تحویل و در نهایت هضم در شرایط روده و معده آزمایشگاهی مورد آزمایش قرار گرفت. روش جذب برای کنترل رهایش ویتامین B در شرایط معده در طول مدت آزادسازی مورد استفاده قرار گرفت. آزمون‌های رهایش ویژگی‌های مفیدی را برای این نوع میکروکپسول مشخص نمود. مکانیسم رهایش با استفاده از مدل‌های سینتیکی پیش‌بینی گردید. نتایح نشاندهنده این بود که اکثر میکروکپسول‌ها به‌صورت کروی با اندازه 100 میکرومتر می‌باشد و این میکروکپسول‌ها به‌ترتیب دارای پایداری بسیار خوب و متوسط در شرایط معده و روده هستند. نتایج همچنین نشان داد که بیشترین میزان رهایش در شرایط معده- روده رخ داده و نوع ویتامین تاثیر اندکی بر میزان رهایش و پروفایل رهایش دارد. مدل‌های سنتیکی پیشنهاد می‌دهد که رهایش ویتامین‌های خانواده B عمدتاً با مکانیسم فیک دیفوزیون رخ می‌دهد. به‌طور کلی، این تحقیق نشان داد که میکروژل آلژینات- پروتئین آب پنیر حاوی ویتامین می‌تواند ویتامین را در برابر هضم معدوی محافظت نموده و به‌عنوان سیستم تحویل استفاده گردد.

کلیدواژه‌ها

• ویتامین B کمپلکس
• رهایش کنترل شده
• میکروژل
• پروتئین آب پنیر
• آلژینات

عنوان مقاله [English]

Vitamin protection by Alginate-Whey Protein Micro Gel (AL-WPC MG) as a novel microcapsule against gastrointestinal condition; case study: B-complex vitamins.

نویسنده [English]

• Mohsen Zandi

Department Food Science and Engineering, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

چکیده [English]

The aim of the current research was to identify and develop an ideal delivery system in order to protect the vitamin from gastrointestinal conditions. For this purpose, vitamin loaded Alginate-Whey protein micro gels (AL-WPC MGs) developed as a biopolymer carrier. This microcapsule was examined in terms of morphology, ζ-potential particle size and distribution, encapsulation and delivery efficiency, and in vitro gastric and intestinal digestions. Absorbance method was used to monitor B-complex vitamins release over time at the simulated gastrointestinal conditions. Release experiments illustrated beneficial attributes for these microspheres. Release mechanism was predicted by using various kinetic equations. Results indicated that the most of the fabricated spherical shaped AL-WPC MGs was under 100 μm in size, and these microcapsules had an excellent and moderate stability in gastric and intestinal conditions, respectively. It was found that the highest vitamin release rate occurs in the simulated gastric-intestinal situation, and type of the vitamin had a slight effect on the release rate and release profile. Kinetic models suggested that release from group B vitamins mainly was controlled by Fickian diffusion mechanism. In general, this research showed that the AL-WPC MGs protect the vitamin from gastric digestion and could be used as a delivery system.
In previous works, a  novel AL-WP MGs and use for different active agent encapsulation was developed, while the final purpose of this work was to study the vitamin release mechanism from AL-WPC MGs at the gastro–intestinal situation. Accordingly, this microcapsule showed the highest vitamin release rate at the simulated intestinal situation. This high release could be due to instability of alginate in neutral pH, and also enzymatic digestion of whey protein. The better release of vitamin at intestinal condition is desirable to achieve the nutrient effect during food consumption. This micro gel therefore appears to be potentially beneficial as digestion delivery vehicles for bioactive compounds in the food and nutraceuticals industry as well as non-food industry.

کلیدواژه‌ها [English]

• B-complex vitamin
• control release
• micro gel
• whey protein
• alginate

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