نوع مقاله : مقاله پژوهشی فارسی

نویسندگان

1 گروه زیست فناوری مواد غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

2 گروه مهندسی آب، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

3 گروه علوم و صنایع غذایی، موسسه غیر انتفاعی بهاران، گرگان، ایران.

چکیده

< p dir="RTL">مطالعه ویژگی‌های پروبیوتیکی و ضدقارچی باکتری‌های اسید لاکتیک جدا شده از بستره‌های تخمیری که فلور میکروبی آن‌ها کمتر مورد مطالعه قرار گرفته است جهت دستیابی به کشت‌های میکروبی پروبیوتیک و محافظت‌کننده از اهمیت به‌سزایی در صنایع تخمیری برخوردار است. در پژوهش حاضر، باکتری اسید لاکتیک غالب پس از تکرار فرایند مایه‌گیری از خمیرترش کینوا، جداسازی و سپس با توالی‌یابی محصولات PCR حاصل از تکثیر توالی هدف مشخص از ژن 16S rDNA آن شناسایی شد. در ادامه، قابلیت‌های پروبیوتیکی جدایه لاکتیکی، شامل مقاومت به اسید و صفرا، اثر ضدباکتریایی، ویژگی‌های خود و دگر اتصالی، حساسیت آنتی‌بیوتیکی و همولیز خون مورد بررسی قرار گرفت. علاوه بر این، اثر بازدارنده جدایه لاکتیکی بر رشد Aspergillus niger نیز به روش کشت دو لایه بررسی شد. توالی‌یابی محصولات PCR منجر به شناسایی Enterococcus hirae به‌عنوان جدایه لاکتیکی غالب خمیرترش کینوا گردید. همچنین این جدایه لاکتیکی پس از تیمار متوالی اسید و صفرا از زنده‌مانی مناسبی برخوردار بود. علاوه بر این، جدایه لاکتیکی بر Bacillus cereus نسبت به سایر عوامل باکتریایی غذازاد اثر بازدارنده بیشتری داشت و تأثیر بازدارندگی روماند خام جدایه لاکتیکی بر عوامل بیماری‌زا به شکل معنی‌داری (05/0p

کلیدواژه‌ها

موضوعات

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