بررسی تأثیر کارواکرول بر ویژگی‌‏های ویژگی فیزیکی، مکانیکی و میکروبی هیدروژل زیستی بر پایه آرد فیلتر حاصل از فرایند آسیابانی گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

• مریم پیروموسوی
• محبوبه کشیری
• یحیی مقصودلو
• مرتضی خمیری
• مهران اعلمی

گروه فناوری مواد غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

https://doi.org/10.22067/ifstrj.v18i1.85240

چکیده

هیدروژل‌ها شبکه سه‌بعدی زنجیره پلیمری با توانایی جذب زیاد آب هستند که جهت کاهش استفاده از منابع فسیلی موردتوجه قرار گرفته است. آرد فیلتر گندم محصولی اجتناب‏‌ناپذیر از طبقه‌بندی آرد تحت سیستم پنوماتیک در صنعت مدرن آسیابانی گندم می‏‌باشد. از عیوب فیلم‏‌های هیدروژلی در صنایع بسته‌‏بندی می‏‌توان به شکنندگی، نفوذپذیری بالا نسبت به آب و مقاومت کم در برابر آب اشاره کرد. از این حیث هدف این پژوهش بررسی اثر غلظت کارواکرول (5 و 10 درصد نسبت به وزن پلیمر) بر ویژگی‌‏های فیزیکی و مکانیکی، ممانعت‌کنندگی، زاویه تماس سطحی و ضدمیکروبی فیلم هیدروژلی بر پایه آرد فیلتر بود. نتایج حاصل از این پژوهش نشان داد با افزایش غلظت کارواکرول در محلول فیلم‌‏سازی، ضخامت و مقاومت به کشش افزایش و رطوبت، حلالیت در آب و کشش‌پذیری فیلم‌های هیدروژلی کاهش یافت. منافد ریز متعددی در سطح فیلم‌های حاوی کارواکرول با استفاده از تصاویر میکروسکوپ الکترونی روبشی موردتأیید قرار گرفت. بیشترین نفوذپذیری نسبت به بخار آب ^10-10× 1/32 گرم مترثانیه پاسکال) و کمترین حلالیت در آب (63/0±01/37 درصد) در فیلم هیدروژلی حاوی 10 درصد کارواکرول مشاهده شد. ارزیابی فعالیت ضدمیکروبی بخارهای کارواکرول فیلم هیدروژلی در محیط جامد نشان داد که اثربخشی بخار این ترکیب علیه آسپرژیلوس نایجر (بازدارندگی کامل رشد) در مقایسه با اشرشیاکلای و لیستریامونوسایتوژنز بیشتر بود. اندیس کاهش لگاریتمی کارواکرول در غلظت 5 درصد در فیلم هیدروژلی بر پایه آرد فیلتر گندم علیه اشرشیاکلای و لیستریامونوسایتوژنز به‌ترتیب 16/1 و 34/1 لگاریتمی بود. بیشترین کاهش اندیس لگاریتمی فیلم فعال هیدروژلی در غلظت 10 درصد کارواکرول علیه لیستریامونوسایتوژنز 71/2 مشاهده شد.

کلیدواژه‌ها

• آرد فیلتر گندم
• بسته‌بندی ضدمیکروبی
• هیدروژل
• کارواکرول

موضوعات

• زیست فناوری مواد غذایی

عنوان مقاله [English]

Investigating the effect of carvacrol on physical, mechanical and antimicrobial properties of bio-hydrogel film based on filter flour obtained from wheat milling process

نویسندگان [English]

• Maryam Moosavi
• Mahboobeh Kashiri
• Yahya Maghsoudlou
• Morteza Khomeiri
• Mehran Alami

Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

چکیده [English]

Introduction: Hydrogels are a three-dimensional network of polymeric matrices with the ability to absorb water through chemical or physical cross-linking. Recently, the development of bio-based hydrogel with the aim of reducing the use of fossil fuel is becoming interested. Wheat filter flour (WFF) is a by-product obtained from air-classification in the modern wheat milling industry. It contains a high level of non-endosperm materials with the ability of water absorption and bio-film making capacity. Nevertheless, hydrogel-based films usually display weak water resistance, flexibility problems, and gas barrier properties. Carvacrol as a phenolic component is used to improve the functional properties of film and reduce the growth of pathogenic and spoilage microorganisms.
 
Materials and Methods: Hydrogel based films were prepared by casting technique. Six grams of WFF was added to 100 ml of distilled water, the pH of the solution was adjusted near to 10.7 with NaOH solution (1 N). The dispersion was heated up to 85°C,  with gentle stirring at 250 rpm for 30 min.Glycerol (35 g/100g of dry polymer) was then added and stirred for another 15 min. Carvacrol, as an active agent incorporated at two concentrations (5 and 10% (g/100g of dry polymer)) and stirred for another 15 min at 40°C and dried in a forced-air oven at 35± 5°C for 24 h. Physical (density, water-solubility, moisture content), mechanical, barrier and antimicrobial properties of active hydrogel-based film were determined. Antimicrobial properties of active hydrogel-based were evaluated in the vapor phase by using the micro- atmosphere method and liquid medium (immersion in broth) as a food model system. Statistical analyses were performed on a completely randomized design with the analysis of variance procedure using SAS software.
 
Results and Discussion: Increasing the carvacrol concentration in the film making solution led to decreasing the water solubility, moisture content, and hydrophobicity properties of WFF based films. The SEM observations confirmed a porous structure of the active hydrogel-based film. The highest water vapor permeability (1.32×10^-10 gm^-1s^-1Pa^-1) and the minimum water solubility (37.01± 0.63%) were observed in hydrogel film with 10% carvacrol. An increase in the concentration of carvacrol produced a greater growth inhibition zone for all microorganisms. The results in vapor phase showed that A.niger exhibited greater sensitivity to carvacrol than other studied bacteria. Hydrogel based films with 5% carvacrol in liquid food model system produced 1.16 and 1.34 log reduction against E. coli and L. monocytogenes, respectively. The greatest antibacterial activity was observed with films containing 10% carvacrol against L. monocytogenes (2.71 log reduction). This work suggested that the WFF hydrogel base film with 10% carvacrol can be used as an active packaging for improving the safety and shelf-life of food products.

کلیدواژه‌ها [English]

• Wheat filter flour
• Agro-industrial by-product
• Carvacrol
• Hydrogel
• Antimicrobial packaging

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