The application of natural ingredients in food formulations plays a key role in public health. The application of natural colorants is helpful in human health. Most of the natural colorants have additional roles such as antioxidant or antimicrobial activities. Natural colorants in foods especially in cake formulations, make an attractive view for Childs. In this study, the effects of three natural colorants of lycopene, chlorophyll, and Berberis Vulgaris extracts were investigated in cupcakes. The amounts of 0, 0.5, 1.5, and 2% of extracts were used. The moisture, volume increment, porosity, color parameters, total phenols, antioxidative effects of extracts, and sensory properties were evaluated. Results showed all-natural colorants increase the final volume, porosity, oxidative stability, phenol content. Also, they reduced the moisture and lipid oxidation of samples. The red, green, and blue parameters of cakes decreased, especially in samples with Berberis Vulgaris extract. The lightness of all samples was significantly reduced after cooking but the lightness of samples with 2% lycopene oleoresin was not high.Sensory evaluation showed the color, flavor, and odor of cakes with lycopene, and chlorophyll oleoresin had the highest scores. The cakes with Berberis Vulgaris extract had the lowest scores in color, flavor, and odor, but the texture and porosity were the same as other samples. Finally, it can be concluded that lycopene oleoresin showed significant acceptance, antioxidative effect, and cake physical properties. Results can be helpful for researches and food industries because the lycopene oleoresin showed a significant antioxidant activity after 3 weeks and the total phenols of Berberis Voulgaris extract showed significant stability after 3 weeks. Especially, the panelists have a significant reflection of the colored food. They propose them as an attractive material for consumption.