فیلم خوراکی زیست‌فعال برپایه کربوکسی‌متیل سلولز حاوی لاکتوباسیلوس کازئی و پروتئین هیدرولیزشده ماهی

نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

• ژیلا قاسمی ¹
• محمد علیزاده خالد آباد ²
• هادی الماسی ³
• مهدی نیکو ⁴

¹ دانشجوی دکترا، دانشگاه ارومیه، دانشکده کشاورزی، گروه علوم و صنایع غذایی

² ارومیه

³ گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

⁴ گروه پاتوبیولوژی و کنترل کیفی، پژوهشکده آرتیمیا و آبزی پروری، دانشگاه ارومیه، ارومیه، ایران

https://doi.org/10.22067/ifstrj.2021.70439.1048

چکیده

به‌دلیل تمایل به غذاهای طبیعی و سالم، بازار غذاهای فراسودمند به‌سرعت در حال رشد است. در این میان، پروبیوتیک‌ها به‌دلیل توانایی بالقوه آن‌ها، در فرمولاسیون‌های غذایی سالم، به‌طور جدی موردتوجه قرار گرفته‌اند. بیشترین نگرانی در مورد پروبیوتیک‌ها این است که ممکن است تعداد باکتری‌های پروبیوتیک در زمان مصرف کمتر از مقدار موردنیاز (CFU/g 10⁷) باشد. بنابراین در این مطالعه، فیلم‌های خوراکی پروبیوتیک کربوکسی‌متیل‌ سلولز (CMC) حاوی لاکتوباسیلوس کازئی و پروتئین هیدرولیزشده عضله ماهی کپور نقره‌ای (SCMH) تهیه شد و زنده‌مانی سلول‌های باکتری در طول 30 روز نگهداری (در فواصل زمانی 1، 10، 20 و 30 روز) در دماهای 25، 4 و 18- درجه سانتی‌گراد بررسی گردید. جهت استخراج پروتئین از روش انحلال قلیایی/ترسیب اسیدی استفاده شد. ایزوله پروتئین استخراجی به‌وسیله آنزیم آلکالاز (%5 وزنی/وزنی) در دمای °Ϲ 50 و 8 = pH به‌مدت 3 دقیقه هیدرولیز گردید. فیلم‌ها با انحلال SCMH و CMC با نسبت 2:1 در آب مقطر تهیه شدند و لاکتوباسیلوس کازئی با غلظت CFU/mL 10⁸ به فیلم‌ها اضافه شد. خصوصیات رنگی، فیزیکی، استحکام کششی نهایی (UTS) و ازدیاد طول در نقطه شکست (EB) فیلم‌ها بررسی شد. الگوهای ساختاری نمونه‌های فیلم با پراش‌سنج اشعه X در دمای اتاق با زاویه پراش (θ2) از 5 تا 40 درجه به‌دست آمد. طیف‌سنجی FT-IR فیلم‌ها در طول موج cm^-1 500-3500 ثبت شد. نتایج آنالیز FT-IR، XRD و DSC، حاکی از شکل‌گیری پیوند هیدروژنی بین لاکتوباسیلوس کازئی و ماتریس فیلم و همچنین اثر پلاستی‌سایزری SCMH بودند. به‌طوری‌که فیلم CMC خالص حاوی باکتری، بالاترین خصوصیات مکانیکی (%9/29=EB، MPa 7/3=UTS) را داشت. افزودن SCMH به فیلم‌ها، به‌طور قابل‌توجهی (p˂0.05) زنده­‌مانی لاکتوباسیلوس کازئی را در همه دماها افزایش داد و توانست در پایان دوره نگهداری در دمای °Ϲ 4 مقدار آن را در حد log CFU/g 01/0 ±7 نگه دارد.

کلیدواژه‌ها

• پروتئین هیدرولیزشده
• لاکتوباسیلوس کازئی
• کربوکسی‌متیل‌ سلولز
• فیلم خوراکی
• پروبیوتیک

موضوعات

• زیست فناوری مواد غذایی

عنوان مقاله [English]

Carboxymethyl cellulose based bioactive edible films with Lactobacillus casei and fish protein hydrolysates

نویسندگان [English]

• Zhila Ghasemi ¹
• Mohammad Alizadeh khaled abad ²
• Hadi Almasi ³
• Mehdi Nikoo ⁴

¹ PhD. Candidate, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran

² urmia

³ Associate Professor, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran

⁴ Associate Professor, Department of Pathobiology and Quality Control, Artemia and Aquaculture Research Institute, Urmia University, Urmia, West Azerbaijan 57179-44514, Iran

چکیده [English]

Nowadays, probiotics have been seriously considered for their potential application in healthy food formulations. The most highlighted concern about probiotics is that the number of probiotic bacteria at the time of consumption may be lower than the required value (10⁷ CFU/g). A new approach is the immobilization of probiotic cells in the matrix of edible films. So in this study, edible carboxymethyl cellulose (CMC) probiotic films containing Lactobacillus casei, and the silver carp muscle protein hydrolysates (SCMH) prepared by using limited Alcalase hydrolysis, were analyzed and the viability of bacterial cells was determined at 25, 4, and -18°C for 30 days. An alkaline solubilization/acid precipitation method was used to isolate silver carp white muscle proteins. Protein isolate (3%, W/V) was hydrolyzed with 5% E/S ratio (w/w) Alcalase at 50°C and pH 8.0 for 3 min. Briefly, films were prepared by dissolving SCMH and CMC powder (1.5%, w/v) in a ratio of 1:2 in distilled water and L. casei was added to a final concentration of 10⁸ CFU/mL. Probiotics were counted at intervals of 1, 10, 20, and 30 day. The physical, mechanical [Ultimate tensile strength (UTS) and elongation at break (EB)], thermal and structural properties were determined. XRD patterns of the film samples collected by X-ray diffractometer (XRD) and Fourier transform infrared (FT-IR) spectroscopy of the film samples were recorded. The results indicated that the addition of SCMH significantly (p˂0.05) improved the L. casei viability at all three temperatures. Thickness, moisture absorption, and water vapor permeability (WVP) of the films were not influenced by addition of the probiotic. However, the addition of SCMH negatively affected the film’s mechanical properties. The FT-IR analysis confirmed the formation of hydrogen bonds between L. casei and the CMC matrix, the XRD and differential scanning calorimetry (DSC) analyses confirmed the plasticizing effect of SCMH on the films. Thus, CMC films containing L. casei showed the highest UTS (3.7 MPa) and EB (29.9%). Generally, the results indicated that the SCMH incorporated CMC-based film can be a good carrier for probiotics as bioactive food packaging system with promising potential for shelf life extension of perishable foods.

کلیدواژه‌ها [English]

• Protein hydrolysates
• L. casei
• Carboxymethyl cellulose
• Edible films
• Probiotic

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