تهیه و ارزیابی ویژگی‌های شناساگر حساس به pH بر پایه فیلم کامپوزیتی صمغ عربی-کربوکسی متیل سلولز حاوی آنتوسیانین ریحان بنفش (Ocimum basilicum. L)

نوع مقاله : مقاله پژوهشی

نویسندگان

• امیر رضائی ¹
• مسعود رضائی ²
• مهدی آل بو فتیله ³

¹ گروه فرآوری محصولات شیلاتی، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس ، نور، ایران

² گروه فرآوری محصولات شیلاتی، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس ، نور، ایران.

³ مرکز ملی تحقیقات فرآوری آبزیان، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندر انزلی، ایران.

https://doi.org/10.22067/ifstrj.2021.69500.1030

چکیده

در این تحقیق، بسته‌بندی هوشمند حساس به pH بر پایه فیلم کامپوزیتی صمغ عربی- کربوکسی متیل سلولز حاوی آنتوسیانین ریحان بنفش (Ocimum basilicum. L) تهیه شد و ویژگی‌های ساختاری، فیزیکی، مکانیکی، حرارتی، آنتی‌اکسیدانی و حساسیت رنگی نسبت به pHهای مختلف و گاز آمونیاک مورد ارزیابی قرار گرفت. بدین منظور ابتدا آنتوسیانین‌های ریحان بنفش استخراج و حساسیت رنگی آن‌ها سنجش گردید. در مرحله بعد آنتوسیانین‌های استخراج شده در سه غلظت 40، 60 و 80 میلی­گرم در 100 میلی‌لیتر به ماتریس پلیمری فیلم کامپوزیت صمغ عربی- کربوکسی متیل سلولز اضافه و ویژگی‌های مختلف آن ارزیابی گردید. نتایج آزمون‌های FTIR و XRD نشان داد که آنتوسیانین استخراج شده به‌خوبی در ساختار فیلم کامپوزیتی صمغ عربی- کربوکسی متیل سلولز قرار گرفته است. اضافه شدن آنتوسیانین به ماتریس پلیمری سبب افزایش نفوذپذیری به بخار آب (g pas^-1 m^-1 s^-1 73/4) و ویژگی‌های آنتی‌اکسیدانی و همچنین کاهش زاویه تماس (33/55 درجه)، کشش‌پذیری (56/1%) و دمای انتقال ذوب گردید. مقاومت کششی فیلم‌های تهیه شده با افزایش میزان آنتوسیانین (صفر تا 60 میلی‌گرم در 100 گرم محلول فیلم) از میزان 19 تا 64/23 مگاپاسکال افزایش یافت اما با افزایش بیشتر میزان آنتوسیانین، کاهش یافت. قرار گرفتن فیلم شناساگر تهیه شده در معرض گاز آمونیاک و pHهای مختلف، منجر به تغییر رنگ فیلم‌ها از رنگ قرمز به رنگ زرد گردید. این تغییرات رنگی فیلم‌ها با تغییرات رنگی محلول آنتوسیانین همخوانی داشت. براساس این نتایج می‌توان اظهار داشت که فیلم شناساگر تهیه شده در این پژوهش می‌تواند به‌عنوان بسته‌بندی هوشمند جهت ارزیابی چشمی تازگی و فساد در محصولات غذایی مورد استفاده قرار گیرد.

کلیدواژه‌ها

• آنتوسیانین
• ریحان بنفش
• صمغ عربی
• فیلم حساس به pH
• کربوکسی متیل سلولز

عنوان مقاله [English]

Preparation and evaluation some properties of pH indicator film based on Arabic gum- Carboxy methyl cellulose composite film containing of Violet basil (Ocimum basilicum. L) Anthocyanin

نویسندگان [English]

• Amir Rezaei ¹
• Masoud Rezaei ²
• Mahdi Alboofetileh ³

¹ Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran.

² Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran.

³ Iran Fish Processing Technology Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran.

چکیده [English]

[1]Introduction: Nowadays, pH indicator has engrossed wide attention because of its eligibility to monitoring shelf life. Intelligent packaging is authoritative of detecting, sensing, tracing, recording and communicating properties to provide information is communicated directly through color changes. In the meantime, natural anthocyanins are non-toxic, water- soluble and safe dye that are indicator to pH change. Anthocyanins are a large group of natural dyes. Some natural anthocyanin, such as anthocyanin extracted from mulberry, black bean, blueberry and violet basil (Ocimum basilicum) are used to spoilage monitoring. In present study, an intelligent pH-indicator film based on Arabic gum- Carboxymethyl cellulose incorporated with Violet basil (Ocimum basilicum. L) Anthocyanin was prepared and its properties including structural, physical, mechanical, thermal, antioxidant activity and color sensitivity to changing pH and ammonia gas were evaluated.
 
Materials and methods: For this purpose, at first anthocyanin of Violet basil was extracted and its color reactions were investigated. In next step, the extracted anthocyanin was added to Arabic gum- Carboxymethyl cellulose at different concentrations (40, 60 and 80 mg/100 g film formation solution) and their properties were evaluated.
 
Results and discussion: The results of FTIR and XRD tests showed that the extracted anthocyanin was successfully dissolved in the composite film. Addition of anthocyanin to film matrix led to increasing the WVP and antioxidant activity and decreasing the water contact angle, elongation at break (EB) and thermal properties. The tensile strength value of the prepared films increased from 19 to 23.64 MPa with increasing anthocyanin content from 0 to 60 mg/100 g film solution; but, it decreased with further increase of the anthocyanin content. Exposing the indicator films to ammonia gas and different pHs led to changing the color changes from red to yellow. These color variations of films were consistent with the color changes of the anthocyanin solution. This result indicates that the films prepared sensitive to pH change, and observed different color in films as the solution is changed from acidic to alkaline because causes its structural changes. The structure of anthocyanin is yellow salt ions in acidic solutions, is converted to quinoidal in weak alkaline solutions, and becomes unstructured in high pH solutions.
In this study, pH indicator films were prepared based of Carboxymethyl cellulose and Arabic gum containing violet basil anthocyanin. The developed films are non-toxic and biodegradable and provide visible color response. Based on these results can be concluded that the indicator film prepared in present study could be used as intelligent food packaging for visual evolution of freshness/spoilage in food products

کلیدواژه‌ها [English]

• Arabic gum
• Carboxy methyl cellulose
• pH indicator film
• Violet basil anthocyanin

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