نوع مقاله : مقاله پژوهشی لاتین

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 گروه زراعت- دانشگاه گرگان

3 دانشگاه صنعتی دانمارک

4 دانشگاه علوم پزشکی گلستان

چکیده

هدف این مطالعه استفاده از واکنش میلارد به‌عنوان ابزاری برای گلیکوزیلاسیون پروتئین‌های هیدرولیزشده حاصل از نخود کاجان (Cajanus cajan) و ارزیابی این اصلاح شیمیایی بر ویژگی‌های آنتی‌اکسیدانی و امولسیفایری بود. ویژگی‌های شیمیایی، ترکیب آمینواسیدی و توزیع وزن مولکولی پروتئین‌های هیدرولیز شده مورد بررسی قرار گرفت. از گلوکز، گالاکتوز و مالتودکسترین در نسبت‌های 1 به 2، 1 به 1 و 2 به 1 (پروتئین هیدرولیزشده به قند، وزن خشک) برای گلیکوزیلاسیون استفاده شد. فعالیت آنتی‌اکسیدانی از طریق دو آزمون فعالیت مهار رادیکال آزاد ‌1و 1- دی‌فنیل-2- پیکریل هیدرازیل (DPPH) و مهار نیتریک‌اکسید بررسی شد. امولسیون‌ها (روغن/ آب) توسط روش سونیفیکاسیون تهیه شدند. توزیع اندازه ذرات و پتانسیل زتا امولسیون‌ها طی 4 روز نگهداری اندازه‌گیری شد. گلیکوزیلاسیون با گلوکز در نسبت 2 به 1 مهار DPPH را از 96/37% به 53/85% و مهار نیتریک‌اکسید را از 50/14% تا 83/54% افزایش داد. همچنین گلیکوزیلاسیون توسط هرکدام از قندها پایداری امولسیون‌ها را افزایش داد و تفاوت معناداری بین نوع قند مورد استفاده مشاهده نشد.

کلیدواژه‌ها

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