نوع مقاله : مقاله پژوهشی فارسی

نویسندگان

1 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه آزاد اسلامی- واحد ورامین پیشوا، ورامین، ایران.

2 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه آزاد اسلامی-واحد ورامین پیشوا، ورامین، ایران

3 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه آزاد اسلامی-واحد ورامین پیشوا، ورامین، ایران.

4 گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.

چکیده

هدف از این تحقیق ریزپوشانی عصاره آبی ریشه گیاه باباآدم و استفاده از آن در نوشیدنی پروبیوتیک بر پایه آب پرتقال- هویج بود. برای این منظور ابتدا عصاره ریشه بابا آدم با دو روش میکروکپسولاسیون و نانوکپسولاسیون و با استفاده از دو ترکیب مالتودکسترین و صمغ عربی به‌عنوان پوشش دیواره، ریزپوشانی گردید. سپس عصاره ریزپوشانی شده (در سطوح 5/0 و 1 درصد) به همراه عصاره آزاد در فرمولاسیون نوشیدنی پروبیوتیک آب پرتقال- هویج استفاده و اثر آن بر زنده‌مانی باکتری‌های پروبیوتیک و همچنین ویژگی‌های فیزیکوشیمیایی و حسی محصول نهایی طی دوره نگهداری 30 روزه در دمای یخچال (°C 5/0± 4) مورد بررسی قرار گرفت. نتایج مرحله ریزپوشانی نشان داد که نانوکپسول‌ها دارای راندمان و محتوای فنلی بالاتری نسبت به میکروکپسول‌ها بودند (05/0>p)، بطوریکه بالاترین راندمان (75/92%) و سطح ترکیبات فنلی (mgGAE/g 385/0) و کوچک‌ترین اندازه ذرات کپسول (22/0± 33/14 میکرومتر) در نانوکپسول‌های تهیه‌شده با صمغ عربی مشاهده شد. تصاویر SEM نیز حاکی از این بود کپسول‌های تولیدی از لحاظ ریزساختار، دارای سطوح شیشه‌ای و زاویه‌دار بوده و شکل منظمی ندارند. با افزودن فرم‌های مختلف عصاره ریشه باباآدم به فرمولاسیون نوشیدنی پروبیوتیک ‌آب پرتقال- هویج حاوی CFU/ml 108 باکتری لاکتوباسیلوس اسیدوفیلوس، مشاهد شد که پارامترهای کیفی از جمله عدد فرمالین، کدورت، ویسکوزیته، فعالیت آنتی‌اکسیدانی و بقاء باکتری‌های پروبیوتیک در نوشیدنی‌ها نسبت به نمونه کنترل افزایش یافت (05/0>p). طی دوره نگهداری در یخچال میزان کدورت، اسیدیته و IC50 در همه تیمارها به‌ویژه در نمونه کنترل افزایش یافت در حالی‌که سایرشاخص‌ها از جمله قابلیت زنده‌مانی باکتری‌های پروبیوتیک روند کاهشی نشان داد. افزودن فرم‌های مختلف عصاره ریشه باباآدم، تأثیر نامطلوبی بر طعم و بوی نمونه‌ها نداشت به‌طوری‌ که کلیه تیمارها قابل پذیرش بودند. بنابراین، بر اساس نتایج این مطالعه می‌توان بیان کرد که عصاره ریشه باباآدم، به‌ویژه در قالب نانوکپسول، می‌تواند برای افزایش ماندگاری پروبیوتیک‌ها و افزایش فعالیت آنتی‌اکسیدانی غذاهای عملگرا طی فرایند تولید و نگهداری مورد استفاده قرار گیرد.

کلیدواژه‌ها

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