افزایش تولید کاروتنوئیدهای ریزجلبک کلرلا با استفاده از تغییر غلظت مواد مغذی و فیتوهورمون

نوع مقاله : مقاله پژوهشی

نویسندگان

• زهره نوروزی مطلق
• محمود اخوان مهدوی
• رضا قشلاقی

گروه مهندسی شیمی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران

https://doi.org/10.22067/ifstrj.2022.79014.1209

چکیده

کاروتنوئیدها اثرات فراوانی بر روی سلامتی انسان دارند. این ترکیبات توسط گیاهان و ریزجلبک­ها تولید می­شوند. امروزه استخراج کاروتنوئیدها از ریزجلبک­ های خوراکی همچون کلرلا بسیار مورد توجه قرار گرفته است، زیرا ریزجلبک‌ها در تمام سال (بدون توجه به فصل) و با سرعتی بسیار بیش­تر از گیاهان در زمین­ های غیر زراعی رشد می­ کنند. هدف از این پژوهش ارایه روشی برای افزایش تولید کاروتنوئیدهای ریزجلبک کلرلا سروکینیانا است. برای این منظور، اثر غلظت­ های متفاوت از دو ماده مغذی نیترات سدیم به‌عنوان منبع نیترات و دی پتاسیم هیدروژن فسفات به‌عنوان منبع فسفات بر روی میزان تولید کاروتنوئیدها به صورت آماری بررسی شد. برای بررسی اثر غلظت­ های مختلف نیترات و فسفات از طرح کاملا" تصادفی آزمایش فاکتوریل و نرم‌افزار Minitab استفاده شد که در آن سطوح غلظت در نظرگرفته شده برای نیترات برابر  04/0، 25/0 و 5/1 و برای فسفات برابر  01/0، 04/0 و 16/0 بود. نتایج نشان داد که در غلظت 5/1 از نیترات و  04/0 از فسفات غلظت کاروتنوئیدها بیشینه است (  21/0  75/6). در این شرایط، جهت افزایش تولید کاروتنوئیدها، به محیط کشت فیتوهورمون نفتالن استیک اسید اضافه شد. در غلظت بهینه ppm 5/2 از نفتالن استیک اسید غلظت کاروتنوئیدها 71/26 % افزایش یافت و به مقدار  15/0  49/8 رسید.

کلیدواژه‌ها

• رنگدانه
• سروکینیانا
• فسفات
• نیترات

موضوعات

• زیست فناوری مواد غذایی

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

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